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English to Chinese: Hydrophobic interaction chromatography
Source text - English Hydrophobic interaction chromatography (HIC) is a versatile liquid chromatography technique which is useful in protein separations, either as a mode of purification or as an analytical tool. The technique can be used alone or in combination with ion exchange chromatography and gel filtration chromatography. HIC is unique in that proteins bind at high salt concentration and elute at low salt concentration. Elution is accomplished with a reverse salt gradient. HIC is sometimes referred to as a milder form of reverse phase chromatography (RPC) since HIC utilizes milder binding and elution conditions than RPC and thus typically retains the biological activity of the target protein. HIC requires minimal sample pre-treatment and can thus be used effectively in combination with traditional protein precipitation techniques. Protein binding to HIC media is promoted by moderately high concentrations of anti-chaotropic salts, which also have a stabilizing influence on protein structure. HIC elution is achieved by a linear or stepwise decrease in the concentration of salt in the adsorption buffer with satisfactory recoveries. As a result, HIC is widely used for purifying a variety of biomolecules, such as serum proteins, membrane-bound proteins, nuclear proteins, receptors, cells, and recombinant proteins in research and industrial laboratories.
Translation - Chinese 疏水作用色谱法（Hydrophobic interaction chromatography；HIC）是一种多功能液体色谱技术，适于以纯化方式或作为分析工具用于蛋白质分离。所述技术可单独使用或与离子交换色谱法和凝胶过滤色谱法组合使用。HIC的独特之处在于蛋白质在高盐浓度下结合并在低盐浓度下洗脱。利用反向盐梯度完成洗脱。HIC有时称为较温和形式的反相色谱法（reverse phase chromatography；RPC），因为HIC所利用的结合和洗脱比RPC更加温和，且因此通常保留靶蛋白的生物活性。HIC需要极少的样品预处理且因此可以有效地与传统蛋白质沉淀技术组合使用。蛋白质与HIC介质的结合通过适当高浓度的抗离液盐促进，所述抗离液盐还对蛋白质结构具有稳定作用。通过吸附缓冲液中盐浓度的线性或逐步降低实现HIC洗脱，回收率令人满意。因此，HIC广泛用于纯化各种生物分子，如血清蛋白、膜结合蛋白、核蛋白、受体、细胞以及研究和工业实验室中的重组蛋白。
Chinese to English: 聚合物 General field: Law/Patents Detailed field: Chemistry; Chem Sci/Eng
Source text - Chinese 本发明涉及—种组合物，其包含两亲物和在20°C下于水中的溶解度不超过10 g/l的活性成分，其中所述两亲物包括与至少一种梳状聚合物连接的超支化聚碳酸酯，其中所述梳状聚合物包含呈聚合形式的聚乙二醇单（甲基）丙烯酸酯或烯丙基醇烷氧基化物，其中所述聚碳酸酯可通过如下方法获得：a) 通过使有机碳酸酯（A）或光气衍生物与具有至少3个羟基的醇（B1）反应而制备缩合产物（K），和 b) 以分子间方式使K转化为所述超支化聚碳酸酯，其中对OH基团与碳酸酯或光气基团的定量比加以选择，以使得K平均具有：i）一个碳酸酯基团或氨基甲酰基团和多于一个的OH基团，或ii）—个OH基团和多于一个的碳酸酯基或氨基甲酰基。
Translation - English The invention relates to a composition comprising amphiphiles and active ingredients which have a solubility of less than 10 g/l in water at 20℃, wherein the amphiphiles include hyperbranched polycarbonate linked to at least one comb-shaped polymers, in which the comb-shaped polymers comprise polyethylene glycol mono(meth)acrylates in polymeric form or allyl alcohol alkoxide, wherein the polycarbonate was obtained by: a) reacting organic carbonate (A) or phosgene derivatives with alcohol having at least three hydroxyl (B) to produce condensation product (K), and b) converting K to the hyperbranched polycarbonate in an intermolecular manner, wherein the quantitative ratio of -OH group to carbonate or phosgene groups was chosen to allow K to have on average: i) one carbonate group or carbamoyl group and more than one -OH group, or ii) one -OH group and more than one carbonate group or carbamoyl.
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Years of experience: 6. Registered at ProZ.com: Feb 2019.
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